4 close centrifuge lid, 5 cooling (only 5424 r), 1 temperature adjustment – Eppendorf 5424 R Centrifuge Manuale d'uso

Pagina 23: 2 temperature display, 3 temperature monitoring, 4 fast temp, Close centrifuge lid, Cooling (only 5424 r) 5.5.1, Temperature adjustment, Temperature display

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23

5 Operation

Centrifuge 5424 / 5424 R — Operating manual

5.4.4

Close centrifuge lid

1. Check correct attachment of rotor and rotor lid.
2. Push down the centrifuge lid until the lid latch engages and the lid is automatically closed.
The centrifuge will close automatically.

The open key lights blue. The display shows the symbol .

5.5

Cooling (only 5424 R)

5.5.1

Temperature adjustment

Set the temperature using the temp arrow keys from -10°C to +40°C.

You can also modify the temperature during centrifugation.

5.5.2

Temperature display

5.5.3

Temperature monitoring

After the set temperature has been reached the centrifuge responds as follows to temperature
fluctuations during centrifugation:

5.5.4

Fast Temp

This function can be used to start a temperature control run directly without samples with a rotor
and temperature-specific speed in order to quickly adjust the rotor chamber up to the set target
temperature.

Requirement

The centrifuge is switched on.

The rotor and rotor lid are properly attached.

The centrifuge lid is closed.

The temperature and g-force (rcf) or speed (rpm) are set for the subsequent centrifugation
(see Centrifuging on p. 24).

1. Press the fast temp key.

The display shows FT as well as the current temperature and g-force (rcf) or speed (rpm).

The cooling time from room temperature (~ 21°C) to 4°C takes approx. 8 min.

The temperature control cycle ends automatically when the target temperature has been
reached. A periodic signal tone sounds.

WARNING!

Centrifuge lid can crush. Keep hands clear.

When opening or closing the device lid, do not reach between the lid and device or into the
latching mechanism of the lid.

Always open the centrifuge lid completely to prevent it from falling.

If the rotor is stopped:

Set temperature

During centrifugation:

Actual temperature

Deviation from the target value

Action

ΔT > 3°C

Temperature display flashes.

ΔT > 5°C

Display Error 18. Centrifugation is stopped
automatically.

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