2 centrifuging in continuous operation, 3 short-spin centrifugation, Centrifuging in continuous operation – Eppendorf 5418 R Centrifuge Manuale d'uso

Pagina 25: Short-spin centrifugation

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Operation

Centrifuge 5418 / 5418 R

English (EN)

25

End of centrifugation

The centrifuge automatically stops after the set time has elapsed. The elapsed centrifugation will be
shown in a blinking display during the braking process. A signal tone sounds when the rotor is at a
standstill.

Only 5418: The centrifuge lid opens automatically. The display shows the symbol .

Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You can open it
by pressing the

open key.

5. Remove the material for centrifuging.

5.4.2

Centrifuging in continuous operation

Perform the following steps in the sequence described.

1. Use

time to set the continuous run.

The continuous run function can be set above 9:59

h or under 30

s (5418) or above 99

min or under

0.5

min (5418 R). The timer shows

oo to indicate continuous operation.

2.

Only 5418 R: Use the temp arrow keys to adjust the temperature.

3. Use

speed to set the g-force

(rcf) or speed

(rpm).

4. Press

start/stop to start centrifuging.

In the display flashes while the rotor is running.
Time is counted upwards, first in 30-second increments and then in minute increments from ten
minutes.

5. Press

start/stop to end centrifuging after the desired time period.

The centrifugation time will be shown in a blinking display during the braking process.

A signal tone sounds when the rotor is at a standstill.

Only 5418: The centrifuge lid opens automatically. The display shows the symbol .

Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You can open it
by pressing the

open key.

6. Remove the material for centrifuging.

5.4.3

Short-spin centrifugation

5418: You can carry out a short run with the maximum g-force

(rcf) / speed

(rpm).

5418 R: You can carry out a short run with the currently set or with the maximum g-force

(rcf)

/

speed

(rpm). Set this short spin mode as described in the following section.

5.4.3.1 Selecting short spin mode (only 5418 R)

1. Press the

short key while the centrifuge lid is open.

The current mode is displayed:

Display

1 – 14 t (run at preselected speed)

Display

14 t (run at maximum speed of 14.000 rpm)

2. When the centrifuge lid is open, press the

short key for longer than 2 s to switch between these modes.

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